Journal: PLoS ONE

Article Title: Anti-TGF-β Antibody, 1D11, Ameliorates Glomerular Fibrosis in Mouse Models after the Onset of Proteinuria

doi: 10.1371/journal.pone.0155534

Figure Lengend Snippet: (A and B) Representative images of NEP25 mouse kidney samples with silver- (A) and PAS staining (B). Disease was induced by injection of LMB2, and 1D11 or 13C4 (control IgG) was administered on day -1 and day 0, followed by further injections every other day until sacrifice at day 28. Scale bar = 100 μm. 40x objective. (C) Glomerular mRNA content was analyzed with laser-captured glomeruli from cryosectioned kidney samples. † P < 0.05 compared to healthy controls by t-test with Welch correction.

Article Snippet: The 1D11 antibody and its isotype-matched murine IgG1 control (13C4) were produced by Genzyme Corporation.

Techniques: Staining, Injection, Control

Journal: PLoS ONE

Article Title: Anti-TGF-β Antibody, 1D11, Ameliorates Glomerular Fibrosis in Mouse Models after the Onset of Proteinuria

doi: 10.1371/journal.pone.0155534

Figure Lengend Snippet: (A) Three days after disease induction, spot urine was tested for proteinuria and mice that were positive of proteinuria were randomly allocated to either 1D11 or 13C4 group and histology was evaluated day 28. Each dot represents the median of the glomerular scar score, which was evaluated by a blinded observer in approximately 80–100 glomeruli of a PAS-stained section. † P < 0.05 compared to healthy controls by Dunn’s post-hoc test compared to no disease controls, following Kruskal-Walis one-way ANOVA. * P < 0.05 compared to LMB2 only group by Mann Whitney test. (B) Glomerular mRNA content was measured with laser-captured glomeruli from cryosectioned kidney samples. † P < 0.05 compared to healthy controls by Bonferroni’s post-hoc test compared to no disease controls, following one-way ANOVA. * P < 0.05 compared to LMB2 only group by unpaired, two-tailed, t -test. (C and D) Representative PAS staining images (C) and immunohistochemical evaluation of type I collagen (D) from the experiment analyzed in A and B are shown. Scale bar = 20 μm. 20x objective. (E) mRNA expression of three TGF-β isoforms in whole kidney of NEP25 mice was analyzed by qPCR. Relative changes compared to day 0 are shown. * P < 0.05 compared to day 0. (F) Mice were injected with 1.0 mg/kg BW of adriamycin via tail vain, and 1D11 administration was started at day 3, followed by every other day, and mice were sacrificed at day 10 for evaluation. Glomerular scar score was evaluated as described for Fig 5A. † P < 0.05 compared to healthy controls. * P < 0.05 compared to LMB2 only group by Mann Whitney test.

Article Snippet: The 1D11 antibody and its isotype-matched murine IgG1 control (13C4) were produced by Genzyme Corporation.

Techniques: Staining, MANN-WHITNEY, Two Tailed Test, Immunohistochemical staining, Expressing, Injection

Journal: PLoS ONE

Article Title: Anti-TGF-β Antibody, 1D11, Ameliorates Glomerular Fibrosis in Mouse Models after the Onset of Proteinuria

doi: 10.1371/journal.pone.0155534

Figure Lengend Snippet: 1D11 prevents podocyte loss (A) WT-1 positive cells numbers per 10 −3 mm 2 were counted in ~100 glomeruli in a PAS-stained kidney section using the TissueGnostics system. Each dot represents the median of WT-1 positivity in a mouse, corrected for a healthy control as 1 from 3 independent experiments. (B) WT-1 positivity was also analyzed with mice that received 1D11 after the onset of proteinuria positive of proteinuria or relevant control mice. Each dot represents median of WT-1-positive cell numbers per 10 −3 mm 2 in glomeruli. † P < 0.05 compared to healthy controls by Dunn’s post-hoc test compared to no disease controls, following Kruskal-Walis one-way ANOVA. Representative images of glomeruli are shown in the bottom panel. WT1: DAB, Nuclei: Hematoxylin. Scale bar = 20 μm. 40x objective. (C) Percentage of pSmad3 positive nuclei over total nucleus numbers per glomerulus was evaluated in ~100 glomeruli per section using the TissueGnositics system. † P < 0.05 by Bonferroni’s post-hoc test compared to healthy controls, following one-way ANOVA. Images taken by Nuance system were remixed for color separation and composite images are shown. Representative images of glomeruli are shown in the bottom panel. pSmad3: DAB, Nuclei: Hematoxylin. Scale bar = 20μm. 40x objective.

Article Snippet: The 1D11 antibody and its isotype-matched murine IgG1 control (13C4) were produced by Genzyme Corporation.

Techniques: Staining, Control

Journal: PLoS ONE

Article Title: Anti-TGF-β Antibody, 1D11, Ameliorates Glomerular Fibrosis in Mouse Models after the Onset of Proteinuria

doi: 10.1371/journal.pone.0155534

Figure Lengend Snippet: Left upper panel: healthy control, right upper panel: LMB2-induced disease mouse. Lower panels: LMB2-induced disease in mice that received 1D11 after detection of proteinuria on day 3. Representative images are shown.

Article Snippet: The 1D11 antibody and its isotype-matched murine IgG1 control (13C4) were produced by Genzyme Corporation.

Techniques: Control